Patients included in this study were allocated, in turn, to three distinct staining methods: 0.05% CV alone, 1% MB alone, or 0.05% CV plus 1% MB (CM double). Each dye (5 mL) was sprayed onto the surface of a rectal polyp and its surrounding normal mucosa through a non-traumatic catheter (Olympus 6233064; Olympus). The mucosal surface was immediately washed with distilled water containing dimethicone, followed by EC of the normal mucosa for 5 min, while keeping the tip of the EC in gentle contact with the surface of the mucosa. Images obtained throughout the procedure were stored on a digital versatile disk (DVD).