All operations were performed under sterile and aseptic conditions. A total of 10 out of 50 female rats were randomly chosen as the sham group and the other 40 rats were established by autologous transplantation to develop the EMs model according to the Vernon and Wilson method with minor modifications. The rats were fed adaptively for 1 week. Modeling: all animals were anaesthetized with an intraperitoneal injection of 1% pentobarbital sodium (35 mg/kg).
Abdominal skin preparation, iodophor disinfection of the abdominal skin, ligation of the left uterus about 1 cm away from the ovary, and uterine horn bifurcation were performed. Next, the endometrium was cut into four pieces of 5 × 5 mm in size, and the endometrial surface was attached to the abdominal wall. The rabbits were sutured with 6-0 absorbable sutures at the four corners of the left and right abdominal walls, while in the control group, only the abdomen was opened and intimal transplantation was not allowed. Four weeks after the first surgery, a second laparotomy was performed to test the success of the model.
Abdominal skin preparation, iodophor disinfection of the abdominal skin, ligation of the left uterus about 1 cm away from the ovary, and uterine horn bifurcation were performed. Next, the endometrium was cut into four pieces of 5 × 5 mm in size, and the endometrial surface was attached to the abdominal wall. The rabbits were sutured with 6-0 absorbable sutures at the four corners of the left and right abdominal walls, while in the control group, only the abdomen was opened and intimal transplantation was not allowed. Four weeks after the first surgery, a second laparotomy was performed to test the success of the model.
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