Transcription Factors in Neural Development

5′capped and polyadenylated mRNAs encoding TFs expressed by cells in the neural plate (foxd4l1.1; Sullivan et al., 2001 (link)), neural crest (foxd3, Sasai et al., 2001 (link); msx1, Suzuki et al., 1997 (link); Tribulo et al., 2003 (link); Monsoro-Burq et al., 2005 (link); zic1, zic2, and zic3, Nakata et al., 1997 (link), Nakata et al., 1998 (link)), PPR (six1;Brugmann et al., 2004 (link)), or epidermis (dlx5, Papalopulu and Kintner, 1993 (link); Luo et al., 2001 (link)), as well as a nucleus-localized β-galactosidase (nβgal) as a lineage tracer, were synthesized in vitro (mMessage mMachine kit, ThermoFisher). Antisense RNA probes for in situ hybridization (ISH) were synthesized in vitro (MEGAscript kit; ThermoFisher) as previously described (Yan et al., 2009 (link)).