Conditional Plp1 Knockout in Mouse Forebrain

To delete the Plp1 gene in the forebrain we interbred Plp^flox mice in which exon 3 of the Plp1 gene is flanked by loxP sites (Lüders et al., 2019 (link); Lüders et al., 2017 (link); Wang et al., 2017 (link)) with mice expressing Cre recombinase under control of the Emx1 gene promoter (Gorski et al., 2002 (link)) on C57Bl/6N background. Genotyping was as reported previously (Gorski et al., 2002 (link); Lüders et al., 2017 (link)). Experimental male Plp^flox/Y*Emx^IREScre and female Plp^flox/flox*Emx^IREScre mice are termed cKO whereas male Plp^flox/Y and female Plp^flox/flox mice served as controls (Ctrl). Mice were bred and kept in the animal facility of the Max Planck Institute of Experimental Medicine with a 12 hr light/dark cycle and two to five mice per cage. All experiments were performed in accordance with the German Animal Protection Law (TierSchG) and approved by the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (LAVES); License numbers were 33.19-42502-04-15/1833 and 33.19-42502-04-18/2803.