Protein Expression Analysis in Cardiac Samples

The homogenization of the samples, protein measurements, electrophoresis, primary and secondary antibody incubation, and quantification were performed following our previously established protocols (Boknik et al. 2019 (link); Gergs et al. 2021 (link); Neumann et al. 2021a (link), 2021b (link)). The following primary antibodies were used: anti-phosphorylated troponin inhibitor (P-TnI; #4004, Cell Signaling Technology Europe, Leiden, Netherlands) and anti-calsequestrin (CSQ) used as loading control (#ab3516, Abcam, Cambridge, UK).

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Neumann J., Azatsian K., Höhm C., Hofmann B, & Gergs U. (2022). Cardiac effects of ephedrine, norephedrine, mescaline, and 3,4-methylenedioxymethamphetamine (MDMA) in mouse and human atrial preparations. Naunyn-Schmiedeberg's Archives of Pharmacology, 396(2), 275-287.

Publication 2022

ab3516

Antibodies Antibody Calsequestrin Electrophoresis Protein Troponin

Corresponding Organization :

Other organizations : Martin Luther University Halle-Wittenberg, University Hospital in Halle

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Variable analysis

independent variables

Homogenization of the samples
Protein measurements
Electrophoresis
Primary and secondary antibody incubation
Quantification

dependent variables

Phosphorylated troponin inhibitor (P-TnI) levels
Calsequestrin (CSQ) levels

control variables

Previously established protocols (Boknik et al. 2019, Gergs et al. 2021, Neumann et al. 2021a, 2021b)
Calsequestrin (CSQ) as a loading control

positive controls

Not specified

negative controls

Not specified

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