Zebrafish Model of E.coli Infection

E.coli infection was performed as previously described (Nguyen-Chi et al., 2014 (link)). In brief, single colony of E.coli which expressing GFP (Olson et al., 2014 (link)) were incubated in LB Broth Miller (MKCL4658; Sigma-Aldrich) containing the antibiotic ampicillin (A100339; Sangon Biotech) at 37°C on orbital shaker for at least 24 h before experimentation. After washing cells in 1×PBS and centrifuging at 500 g for 5 min, the E.coli were resuspended and diluted to the desired concentration in 1×PBS. E.coli were injected into the otic vesicle of zebrafish larvae and observed at the desired developmental stage.