Photoautotrophic Growth of Cr CC1690 Cells

Cr CC1690 cells were grown photoautotrophically in TP^{62 (link)}, TP10, or B&D medium^{63 (link)} at 25 °C, and the illumination of 125 μmol m⁻² s⁻¹ under continuous light conditions. Cultures were kept in a rotatory shaker at 70 RPM. Cells in the mid-logarithmic phase were used as inocula for the different experiments. Cell growth was determined either by measuring samples in a Multisizer 4e Coulter counter (Beckman Coulter Inc., California, USA) particle counter with the Beckman Coulter Multisizer software (v4.03) or using an Infinite M200Pro (TECAN Austria GmbH, Grödig, Austria) plate reader with the TECAN i-control software (v2.0.10.0), to determine either absorbance at 750 nm or chlorophyll fluorescence (excitation 440/9 nm, emission 680/20 nm).

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Durán P., Flores-Uribe J., Wippel K., Zhang P., Guan R., Melkonian B., Melkonian M, & Garrido-Oter R. (2022). Shared features and reciprocal complementation of the Chlamydomonas and Arabidopsis microbiota. Nature Communications, 13, 406.

Publication 2022

Multisizer 4e Coulter counter Infinite M200Pro

Cell Chlorophyll Fluorescence Light

Corresponding Organization :

Other organizations : Max Planck Institute for Plant Breeding Research, Cluster of Excellence on Plant Sciences

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Variable analysis

independent variables

Growth medium (TP^62, TP10, or B&D medium^63)

dependent variables

Cell growth
Absorbance at 750 nm
Chlorophyll fluorescence (excitation 440/9 nm, emission 680/20 nm)

control variables

Temperature (25 °C)
Illumination (125 μmol m^-2 s^-1 under continuous light conditions)
Shaker speed (70 RPM)

controls

Cells in the mid-logarithmic phase were used as inocula for the different experiments.

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