Transgenic Mice Model for Metabolic Regulation

Male C57BL/6 wild‐type mice (4 weeks old, weighing 15 ± 3 g, SPF grade) were purchased from Chongqing Enswell Biotechnology Co., LTD (Chongqing, China). All protocols in this study were approved by the Laboratory Animal Care Committee of Three Gorges Hospital, affiliated with Chongqing University. The mice were fed adaptively for 2–12 h, alternating day and night, temperature of 20–22°C, humidity of 40%–60%, drinking water freely and eating ordinary feed. After 1 week, 32 mice were randomly divided into wild‐type general feeding, wild‐type high‐fat diet, JAZF‐1 transgenic general feeding and JAZF‐1 transgenic high‐fat diet groups (n = 8). The weight and energy compositions between the general diet and high‐fat diet are shown in Table 1. Transgenic mice overexpressing JAZF‐1 were obtained by continuously injecting AAV‐JAZF‐1 in their tail vein for 3 days. Another 32 mice were injected with AAV‐JAZF‐1 and randomly assigned to general feeding plus normal saline, high‐fat diet plus normal saline, general feeding plus PPAR‐γ inhibitor (GW9662) and high‐fat diet plus PPAR‐γ inhibitor (n = 8). The insulin resistance model was constructed using a high‐fat diet, while an ordinary diet was given to the control group, and the body weight of mice was recorded weekly.