Fifty endometrial biopsies from patients with a clinical diagnosis of AUB were taken. Endometrial biopsies were subjected to routine histopathological processing and H and E stain. Endometrium in proliferative phase, secretory phase, endometrial polyps, disordered proliferative endometrium with stromal breakdown/anovulatory pattern were studied for the presence of plasma cells. Proliferative endometrium was further divide into proliferative endometrium with breakdown (PEB) and normal/inactive proliferative endometrium (PP). DPE was used to describe biopsies with irregulary spaced and dilated glands often accompanied with stromal breakdown. Stromal breakdown included presence of clustered neutrophils in the stroma with stromal cell apoptosis. Stromal hemorrhage with fragmentation alone, stromal edema or a loosely packed stroma was considered insufficient for stromal breakdown. Endometrial hyperplasia and malignancy were excluded from the study.
Endometrial biopsy were subjected to IHC, were cut and mounted on 3-aminopropyl- trethoxysilane coated slides, and stained using syndecan-1 (monoclonal mouse antihuman CD 138; Clone M115, Dako). Plasma cells were identified on H and E and Syndecan-1 staining. The immunostained slides were scored for the presence of plasma cells using light microscopy, in at least 10 high power fields. The biopsy was graded as “Negative” - when no plasma cells stained with syndecan, 1+ when <5 plasma cells were present, 2+ when 5-10 plasma cells were present, 3+ when >10 plasma cells were present.
The secondary histologic features of chronic endometritis like gland architectural irregularity, spindled stroma, stromal edema and stromal hemorrhage with the presence of plasma cells was statistically analysed using SPSS software version 14 and Fischer's exact test was analysed. Values of P < 0.05 were considered as significant.
Endometrial biopsy were subjected to IHC, were cut and mounted on 3-aminopropyl- trethoxysilane coated slides, and stained using syndecan-1 (monoclonal mouse antihuman CD 138; Clone M115, Dako). Plasma cells were identified on H and E and Syndecan-1 staining. The immunostained slides were scored for the presence of plasma cells using light microscopy, in at least 10 high power fields. The biopsy was graded as “Negative” - when no plasma cells stained with syndecan, 1+ when <5 plasma cells were present, 2+ when 5-10 plasma cells were present, 3+ when >10 plasma cells were present.
The secondary histologic features of chronic endometritis like gland architectural irregularity, spindled stroma, stromal edema and stromal hemorrhage with the presence of plasma cells was statistically analysed using SPSS software version 14 and Fischer's exact test was analysed. Values of P < 0.05 were considered as significant.
