Filter Paper Insect Repellent Assay

Whatman No. 1 filter papers were cut into rectangles of size 14.7 cm × 17.5 cm. Separate papers were treated with each binary mixture formulation using a calibrated micropipette delivering 2.8 mL of solution. Untreated control papers were treated with absolute ethanol only. DEET (2.5% v/v) was used as a gold-standard repellent. The treated papers were placed on a plastic sheet and air-dried at room temperature for at least 1 h before use. Multiple papers in each formulation were discarded after being used once [17 (link),21 (link)].

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Panthawong A., Nararak J., Jhaiaun P., Sukkanon C, & Chareonviriyaphap T. (2023). Synergistic Behavioral Response Effect of Mixtures of Andrographis paniculata, Cananga odorata, and Vetiveria zizanioides against Aedes aegypti (Diptera: Culicidae). Insects, 14(2), 155.

Publication 2023

No. 1 filter papers

Absolute ethanol Deet Gold

Corresponding Organization : The Royal College Of Anesthesiologists Of Thailand

Other organizations : Walailak University

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Variable analysis

independent variables

Binary mixture formulations

dependent variables

Repellent efficacy

control variables

Size of Whatman No. 1 filter papers (14.7 cm × 17.5 cm)
Volume of solution applied (2.8 mL)
Air-drying time (at least 1 h)

controls

Positive control: DEET (2.5% v/v)
Negative control: Absolute ethanol

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