Protein Expression Analysis by Western Blot

Protein extraction, two-dimensional polyacrylamide gel electrophoresis, and transfer to nitrocellulose membrane were carried out as previously described [11 (link),12 (link),15 (link)]. The membrane was incubated with the following antibodies: cleaved CASP3, cleaved PARP, PARP, p-EGFR, EGFR, p-AKT, AKT, p-ERK, ERK, p-STAT3, STAT3, eIF4A3, p-4E-BP1, mTOR, p-P70-S6, MUC1, and MUC1-C, purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody to glyceraldehyde-3-phosphate dehydrogenase was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

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Takahashi S., Noro R., Seike M., Zeng C., Matsumoto M., Yoshikawa A., Nakamichi S., Sugano T., Hirao M., Matsuda K., Hamada M, & Gemma A. (2021). Long Non-Coding RNA CRNDE Is Involved in Resistance to EGFR Tyrosine Kinase Inhibitor in EGFR-Mutant Lung Cancer via eIF4A3/MUC1/EGFR Signaling. International Journal of Molecular Sciences, 22(8), 4005.

Publication 2021

cleaved CASP3 cleaved PARP p-EGFR p-AKT p-ERK p-STAT3 STAT3 eIF4A3 p-4E-BP1 p-P70-S6 MUC1-C antibody to glyceraldehyde-3-phosphate dehydrogenase

4e bp1 Antibodies Antibody Casp3 Egfr Glyceraldehyde 3 phosphate dehydrogenase Membrane Mtor Muc1 Nitrocellulose Protein Stat3 Two dimensional polyacrylamide gel electrophoresis

Corresponding Organization : Nippon Medical School

Other organizations : Waseda University

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Variable analysis

dependent variables

Cleaved CASP3
Cleaved PARP
P-EGFR
P-AKT
P-ERK
P-STAT3
STAT3
EIF4A3
P-4E-BP1
P-P70-S6
MUC1-C

control variables

Glyceraldehyde-3-phosphate dehydrogenase

controls

No positive or negative controls were explicitly mentioned

Annotations

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