Visualizing VIP neurons in mice

All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with NIH guidelines for the care and use of laboratory animals. Animals were kept on a 12-hour day/night cycle with ad libitum access to food and water. To visualize VIP neurons, VIP-IRES-Cre mice (VipTM1(cre)Zjh/J, Jackson Laboratory, stock #010908) (Taniguchi et al., 2011 (link)) were crossed with Ai14 reporter mice (B6.Cg-Gt(ROSA)26SorTM 14(CAG–tdTomato)Hze/J, Jackson Laboratory, stock #007914) (Madisen et al., 2010 (link)) so that the fluorescent protein tdTomato was expressed only in VIP neurons (Beebe et al., 2022 (link)). Since mice on the C57BL/6J background undergo age-related hearing loss after 3 months of age (Zheng et al., 1999 (link)), all experiments were performed on mice between postnatal days (P)30 – 66.

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Drotos A.C., Herrera Y.N., Zarb R.L, & Roberts M.T. (2023). GluN2D-containing NMDA receptors enhance temporal integration in VIP neurons in the inferior colliculus. bioRxiv.

Publication Preprint 2023

VipTM1(cre)Zjh/J B6.Cg-Gt(ROSA)26SorTM 14(CAG–tdTomato)Hze/J

Age related hearing loss Animals C57bl mice Food Institutional animal care and use committee Ires Laboratory animals Mice Neurons Protein Rosa Tdtomato

Corresponding Organization : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Genotype (VIP-IRES-Cre mice crossed with Ai14 reporter mice)

dependent variables

Expression of fluorescent protein tdTomato in VIP neurons

control variables

Age of mice (postnatal days 30-66)
Housing conditions (12-hour day/night cycle, ad libitum access to food and water)

controls

Positive control: VIP-IRES-Cre mice crossed with Ai14 reporter mice to visualize VIP neurons
Negative control: Not explicitly mentioned

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