Monocyte Isolation from Whole Blood

Whole blood was collected into sodium-heparin containing blood collection tubes (Becton Dickinson) and processed with 4–6 h after collection. The methods used in monocyte isolation have been previously described (Fairfax et al., 2014 (link)) but briefly, we isolated peripheral blood mononuclear cells by density gradient centrifugation of blood-diluted with Hanks Buffered Saline solution (HBSS, Life Technologies, UK) layered on Lymphoprep (Axis-Shield, Norway), and sorted CD14 + monocytes using magnetic-activated cell sorting (MACS, Miltenyi Biotech). The CD14- fraction was used in recreating ML ratios after counting cells by microscopy.

Free full text: Click here

Naranbhai V., Fletcher H.A., Tanner R., O'Shea M.K., McShane H., Fairfax B.P., Knight J.C, & Hill A.V. (2015). Distinct Transcriptional and Anti-Mycobacterial Profiles of Peripheral Blood Monocytes Dependent on the Ratio of Monocytes: Lymphocytes. EBioMedicine, 2(11), 1619-1626.

Publication 2015

blood collection tubes HBSS Lymphoprep MACS

Axis Blood Density gradient centrifugation Hbss Isolation Lymphoprep Microscopy Monocytes Peripheral blood mononuclear cells Saline solution Sodium heparin

Corresponding Organization :

Other organizations : Wellcome Centre for Human Genetics, University of Oxford, Jenner Institute, London School of Hygiene & Tropical Medicine

Top 5 similar protocols

Variable analysis

independent variables

Monocyte isolation method

dependent variables

Monocyte count

control variables

Time between blood collection and processing (4-6 hours)
Sodium-heparin blood collection tubes
Density gradient centrifugation using Lymphoprep
Magnetic-activated cell sorting (MACS) for CD14+ monocyte isolation
Cell counting by microscopy

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!