The coding sequences of CML12, MLO4 and MLO4C were amplified by PCR using the primers XbaI-CML12/CML12-BamHI, XbaI-MLO4/MLO4-BamHI, and XbaI-MLO4C/MLO4-BamHI (Table S2 ). Applying the biolistic PDS-1000/He gene gun system (Bio-Rad, http://www.biorad.com/ , accessed on 11 July 2020), onion epidermal cells were transiently transformed. After cultivated on 1.5 MS culture medium (1.5% Agar, 1/2 MS) for 16–24 hours, the fluorescent signals were detected under a Leica confocal laser scanning microscope (LSM710, Carl Zeiss, http://www.zeiss.com/ , accessed on 21 March 2020) as described by Gookin et al [56 (link)].
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