Oxidative stress modulation in HT22 cells

HT22 cells were seeded into six-well cell culture plates and divided into four groups: control, tempol alone (3 mM in media), BSO alone (1 mM in media), and BSO + tempol. BSO was purchased from Sigma-Aldrich (St. Louis, MO) and tempol was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Dose of BSO (1 mM) was selected based on our previously published data [16 (link)]. Cells were treated with BSO or vehicle (DMEM media) when 60–70% are confluent. tempol was added 10 h after addition of BSO. Cells were trypsinized at 14 h and used for various analyses. Thus, they are control (no treatment at 0 h, trypsinized at 14 h), BSO (1 mM BSO added at 0 h, trypsinized at 14 h), tempol (no treatment at 0 h, 3 mM tempol added at 10 h and trypsinized at 14 h), and BSO + tempol (1 mM BSO added at 0 h, 3 mM tempol added at 10 h and trypsinized at 14 h). All experiments were conducted at least 3-4 times.