Evaluating MSC-Derived Exosome Effects

HaCaT and HDFs cells were cultured until 70~80% confluence, and trypsinized cells were plated in 96-well plates at a density of 4000 cells per well. Briefly, hBM-MSC-Ex purification and characterization were as per previously published methods [8 (link)]. The cells were treated with either hBM-MSC-Ex (25 μg/mL) or PBS (control) (Invitrogen, Shanghai, China), then followed by incubated at 37 °C with 5% CO₂ for 5 days. The cell viability was determined by 10% CCK-8 solution (Sigma, San Francisco, USA), the cultures were incubated for 30 min at 37 °C in 5% CO₂, and corresponding OD value was measured at the 490-nm wavelength.