Linezolid-resistant E. faecalis Isolates

A panel of 44 linezolid-resistant E. faecalis isolates was obtained from the First Affiliated Hospital of Chongqing Medical University, Chongqing, China (Hua et al., 2019 (link)). All isolates showed minimum inhibitory concentrations (MICs) > 4 mg/L of linezolid as determined by the broth microdilution method following the Clinical and Laboratory Standards Institute (CLSI, 2018 ) recommendations. The drug resistance phenotypes and multi-locus sequence types (MLST) of all these isolates have been determined in our previous study (Hua et al., 2019 (link); Table 1 and Supplementary Table S1). The E. faecalis ATCC 29212 isolate obtained from the American Type Culture Collection (Manassas, VA, United States) served as a quality control strain. E. faecalis JH2-2 isolate was kindly provided by Dr. Tieli Zhou (The First Affiliated Hospital of Wenzhou Medical University, China) and used as the recipient strain in conjugation experiments.

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Zou J., Tang Z., Yan J., Liu H., Chen Y., Zhang D., Zhao J., Tang Y., Zhang J, & Xia Y. (2020). Dissemination of Linezolid Resistance Through Sex Pheromone Plasmid Transfer in Enterococcus faecalis. Frontiers in Microbiology, 11, 1185.

Publication 2020

E. faecalis ATCC 29212

Clinical laboratory Drug resistance Linezolid Minimum inhibitory concentrations Phenotypes Strain

Corresponding Organization : Chongqing Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Minimum inhibitory concentrations (MICs) of linezolid

control variables

Broth microdilution method following the Clinical and Laboratory Standards Institute (CLSI, 2018) recommendations
E. faecalis ATCC 29212 isolate obtained from the American Type Culture Collection (Manassas, VA, United States) as a quality control strain
E. faecalis JH2-2 isolate provided by Dr. Tieli Zhou (The First Affiliated Hospital of Wenzhou Medical University, China) used as the recipient strain in conjugation experiments

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