Polymorphisms of the FCN2 gene: − 64 A > C (rs78654553), (located within promoter), + 6,359 C > T (rs175491193), and + 6,424 G > T (rs7851696) (both exon 8) were analysed using allele-specific PCR, as described previously64 (link). Another promoter polymorphism, − 4 A > G (rs17514136) was investigated using PCR–RFLP procedure, employing MboII restriction enzyme (Thermo Fisher Scientific)64 (link).
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