Maintenance of Toxoplasma Gondii Strains

All parasite strains, including parental RHΔhxgprt, TgSKP1^SF [20 (link)], TATiΔku80 [67 (link)] and RHΔhxgprtΔku80) strains [68 (link)], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR), 2 mM L-glutamine (VWR), and 100 IU/ml penicillin– 100 μg/ml streptomycin (VWR). Parasites were released from host cells by passage through a 27-gauge needle [69 (link)]. All parasite strains and host cell lines were routinely tested for Mycoplasma contamination with the MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland) and found to be negative.

Free full text: Click here

Baptista C.G., Lis A., Deng B., Gas-Pascual E., Dittmar A., Sigurdson W., West C.M, & Blader I.J. (2019). Toxoplasma F-box protein 1 is required for daughter cell scaffold function during parasite replication. PLoS Pathogens, 15(7), e1007946.

Publication 2019

DMEM fetal bovine serum L-glutamine penicillin streptomycin MycoAlert Mycoplasma Detection Kit

Cell lines Cells Eagle Fetal bovine serum Fibroblasts Foreskin Glutamine Human Mycoplasma Needle Parasite Parental Penicillin Strains Streptomycin

Corresponding Organization : University of Georgia

Top 5 similar protocols

Variable analysis

independent variables

Parasite strains, including parental RHΔhxgprt, TgSKP1SF [20], TATiΔku80 [67], and RHΔhxgprtΔku80 [68] strains

dependent variables

Not explicitly mentioned

control variables

Human foreskin fibroblasts (ATCC; Manassas, VA) used as host cells
Dulbecco's Modification of Eagle's Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR), 2 mM L-glutamine (VWR), and 100 IU/ml penicillin– 100 μg/ml streptomycin (VWR) used as growth medium
Parasites were released from host cells by passage through a 27-gauge needle [69]
All parasite strains and host cell lines were routinely tested for Mycoplasma contamination with the MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland) and found to be negative

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!