Pseudomonas Bacterial Motility Study

We used Pseudomonas fluorescens CHA0 (wild type)37 (link) and Pseudomonas aeruginosa MPAO1 (wild type) (P. aeruginosa Mutant Library - University of Washington) bacterium for this study. The bacteria strain from −80 °C collection was incubated overnight in the Luria – Bertani (LB) agar plate at 30 °C. One single colony was taken from the agar plate and poured in Luria – Bertani (LB) broth. P. fluorescens was incubated in this media for 14 hours in shaking incubator (Fisher Scientific, Canada) at 30 °C and 150 rpm and P. aeruginosa was incubated in this media for 1 hour and 20 minute in shaking incubator at 37 °C and 150 rpm. Prior to injection into the microfluidic device 200 nm red fluorescent amine-coated polystyrene particles were mixed with the bacteria solution in volume percent concentration of 0.04% (v/v).