Microarray Analysis of Gene Expression

Individual RNA samples were analyzed using Affymetrix GeneChip Mouse Gene 2.0 ST Arrays (Cat. 902118, Thermo Fisher Scientific Inc., Waltham, MA) and GeneChip WT PLUS Reagent Kit (Cat. 902280, Thermo Fisher Scientific Inc., Waltham, MA) according to the manufacturer’s instructions. Microarray data analysis was performed using the Rosetta Resolver software system (Rosetta Bio Software, Seattle, WA). Significantly different mRNA levels were defined using the following criteria: one-way ANOVA with Benjamini and Hochberg FDR (p ≤ 0.05), signal correction statistics (Ratio Builder software, Rosetta Resolver) (p ≤ 0.05), and an expression value ratio between the different conditions of 1.5-fold^{10 (link),22 (link)}.

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Glaubitz J., Wilden A., Golchert J., Homuth G., Völker U., Bröker B.M., Thiele T., Lerch M.M., Mayerle J., Aghdassi A.A., Weiss F.U, & Sendler M. (2022). In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response. Nature Communications, 13, 4502.

Publication 2022

Affymetrix GeneChip Mouse Gene 2.0 ST Arrays GeneChip WT PLUS Reagent Kit

Anova Gene Genechip Microarray analysis Mouse Mrna

Corresponding Organization :

Other organizations : Universitätsmedizin Greifswald, Universität Greifswald, LMU Klinikum, Ludwig-Maximilians-Universität München

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Variable analysis

independent variables

Different experimental conditions

dependent variables

MRNA levels

control variables

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controls

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