High-throughput Compound Screening in Flies

U-GLAD system was utilized for large-scale compound screening in flies [19 ]. In brief, a compound library containing 1508 FDA approved drugs and 345 natural products (DiscoveryProbe^™ FDA-approved Drug Library, ApexBio Cat# L1001) were individually mixed in Gum Arabic and dissolved in chemically defined liquid food to form micelles (final concentration: 5 mg/ml). These micelles are then delivered to CRE-LUC flies in vials by a U-shape glass capillary. Chemically defined food recipe was based on previous study [37 (link)]. CRE-Luciferase activity was measured using the Steady-Glo Luciferase Assay Kit (Promega). Protein concentrations were determined with BSA as a standard using a bicinchoninic acid (BCA) protein determination kit (YEASEN Cat#20201ES76) according to the manufacturer’s instructions.