Volatiles were collected at different time intervals after culture inoculation (1, 4, 8, and 12 days) using polydimethylsiloxane (PDMS) sorbent [48 (link)]. Two to three 5 mm PDMS tubes were carefully mounted on sterile metal wires imbedded in the PDA. The headspace sampling time was 2 h for all experiments unless otherwise indicated. After sampling, the tubes were placed in 1.5 mL brown glass vials and stored at −20 °C for analysis.
Volatiles collected on PDMS tubes were analyzed using a GC-2010 plus gas chromatograph coupled to a MS-QP2010 quadrupole mass spectrometer equipped with a TD-20 thermal desorption unit (Shimadzu, Japan) and a GC Cryo-Trap (Tenax®). A single tube was placed in a 89 mm glass thermal desorption tube and desorbed at a flow rate of 60 mL min−1 for 8 min at 200 °C under a stream of N2 gas. The desorbed substances were focused in a cryogenic trap at −60 °C. The Tenax® adsorbent was heated to 230 °C and the analytes were injected using split mode (1:50) onto a Rtx-5MS GC column (thickness—0.25 µm, length—30 m, film diameter—0.25 µm) using helium as carrier gas. Separation, detection, and data analysis were identical to those reported for the quantitative analysis.
Volatiles collected on PDMS tubes were analyzed using a GC-2010 plus gas chromatograph coupled to a MS-QP2010 quadrupole mass spectrometer equipped with a TD-20 thermal desorption unit (Shimadzu, Japan) and a GC Cryo-Trap (Tenax®). A single tube was placed in a 89 mm glass thermal desorption tube and desorbed at a flow rate of 60 mL min−1 for 8 min at 200 °C under a stream of N2 gas. The desorbed substances were focused in a cryogenic trap at −60 °C. The Tenax® adsorbent was heated to 230 °C and the analytes were injected using split mode (1:50) onto a Rtx-5MS GC column (thickness—0.25 µm, length—30 m, film diameter—0.25 µm) using helium as carrier gas. Separation, detection, and data analysis were identical to those reported for the quantitative analysis.
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