Western Blot Analysis of BATF2 in Sarcoma

Western blot was performed as we described (24 (link)). Briefly, proteins were extracted from sarcoma cells and tissues by using RIPA lysis buffer (Beyotime). Proteins were separated and transferred onto PVDF membranes, which were then incubated with the antibodies against BATF2 (Abcam, Cambridge, MA, USA) or β-actin (Santa Cruz, Dallas, TX, USA), followed by the incubation of secondary antibody. Gray-scale of the bands were quantified by using ImageJ software.

Free full text: Click here

Xu W., Huang Y., Lei Z, & Zhou J. (2024). miR-939-3p induces sarcoma proliferation and poor prognosis via suppressing BATF2. Frontiers in Oncology, 14, 1346531.

Publication 2024

RIPA lysis buffer BATF2 β-actin

Corresponding Organization : Southwest Hospital

Other organizations : Wuhan Third Hospital, Tongren Hospital, Xinqiao Hospital

Top 5 similar protocols

Variable analysis

independent variables

Sarcoma cells and tissues

dependent variables

Expression of BATF2 protein

control variables

Use of RIPA lysis buffer to extract proteins
Separation and transfer of proteins onto PVDF membranes
Incubation with antibodies against BATF2 and β-actin
Incubation with secondary antibody
Quantification of band intensity using ImageJ software

controls

Positive control: Incubation with antibody against β-actin
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!