Images were obtained with a Nikon SMZ1500 microscope or a Nikon inverted A1r spectral confocal microscope. Confocal z-stack image acquisition in multiple fluorescent channels was used to examine the entire dorsal aorta in the AGM . Immunofluorescence double staining was performed as described previously,58 (link) with anti-p-akt S473 (1:25; cat. no. 9271; Cell Signaling) and AlexaFluor 594–conjugated anti-rabbit secondary antibody (1:1000; cat. no. A11012; Life Technologies).
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Confocal Imaging of Dorsal Aorta
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Other organizations : University of Geneva, University of Birmingham, Vlaams Instituut voor Biotechnologie, Ghent University
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- Microscope used: Nikon SMZ1500 microscope or Nikon inverted A1r spectral confocal microscope
- Examination of the entire dorsal aorta in the AGM
- Immunofluorescence double staining was performed as described previously
- Anti-p-akt S473 antibody (1:25; cat. no. 9271; Cell Signaling)
- AlexaFluor 594–conjugated anti-rabbit secondary antibody (1:1000; cat. no. A11012; Life Technologies)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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