Sampling Tissues and Cell Lines for Genomic Analysis

We sampled tissues (liver and blood cells) from a female purchased from a local farmer in Japan because the previous whole-genome sequencing used the whole blood of a female [26 (link)]. All experiments were conducted in accordance with the Guideline of the Institutional Animal Care and Use Committee of RIKEN Kobe Branch (Approval ID: A2017–12).
The human lymphoblastoid cell line GM12878 (Coriell Cat# GM12878, RRID:CVCL_7526) was purchased from the Coriell Cell Repositories and cultured in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 15% fetal bovine serum, 2 mM L-glutamine, and a 1× antibiotic-antimycotic solution (Thermo Fisher Scientific), at 37 °C, 5% CO₂, as described previously [37 (link)].