Unless otherwise noted, all chemicals used in this study were obtained from Sigma-Aldrich, Carbosynth, or GE Healthcare Biosciences. The bacterial growth medium, agarose and isopropyl-β-d -thiogalactopyranoside (IPTG) were purchased from Research Products International (RPI). Escherichia coli strain BL21-Gold (DE3) and the restriction enzyme DpnI were obtained from New England Biolabs. PfuTurbo DNA polymerase was purchased from Agilent. DNA sequencing was conducted by Eton Biosciences Inc. GDP-d -glycero-α-d -manno-heptose (1 ) was enzymatically synthesized and purified as described previously (9 (link)).
Protocol detail
Enzymatic Synthesis of GDP-Glycero-Manno-Heptose
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Variable analysis
independent variables
- Chemicals used in the study
- Bacterial growth medium
- Agarose
- Isopropyl-β-D-thiogalactopyranoside (IPTG)
- Escherichia coli strain BL21-Gold (DE3)
- Restriction enzyme DpnI
- PfuTurbo DNA polymerase
- GDP-D-glycero-α-D-manno-heptose (1)
- DNA sequencing
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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