Recombinant reference allergens (Bet v 1.0101, Bet v 1.0102, Aln g 1.0101, Cor a 1.0104, Car b 1.0109 and Que a 1.0301) were produced according to published methods [9 (link), 10 (link)] and characterized physico-chemically [6 (link)]. Endotoxin content was < 0.3 ng/mg for Bet v 1.0101, < 0.3 ng/mL for Bet v 1.0102, < 0.3 ng/mL for Aln g 1.0101, 0.8 ng/mL for Cor a 1.0104, < 0.3 ng/mg for Car b 1.0109, and < 0.3 ng/mg for Que a 1.0301 as determined by limulus amoebocyte lysate (LAL) assay (Associates of Cape Cod, Inc., East Falmouth, MA, USA).
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Recombinant Reference Allergen Production
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Corresponding Organization : Christian Doppler Klinik
Other organizations : Medical University of Vienna, Christian Doppler Laboratory for Thermoelectricity, Istituti di Ricovero e Cura a Carattere Scientifico
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Variable analysis
independent variables
- Recombinant reference allergens (Bet v 1.0101, Bet v 1.0102, Aln g 1.0101, Cor a 1.0104, Car b 1.0109 and Que a 1.0301)
- Endotoxin content
- Limulus amoebocyte lysate (LAL) assay (Associates of Cape Cod, Inc., East Falmouth, MA, USA)
- No positive or negative controls were explicitly mentioned.
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