Nile Red (Thermo Fisher Scientific) was used to visualize differences in lipid stores of adult females (6–9 days old) reared under diapause (19 °C, 9:15 L:D) and control conditions (25 °C, 16:8 L:D). Fat bodies were dissected in 4% paraformaldehyde and stained using a 1:100 PBS dilution of 500 μg/ml Nile Red powder in acetone stock as previously described18 (link),31 (link). After incubating tissues for 10 min, samples were imaged using a Nikon 50i fluorescent microscope and processed with Nikon imaging software (NIS Elements D 3.2).
Field E.N., Shepard J.J., Clifton M.E., Price K.J., Witmier B.J., Johnson K., Boze B., Abadam C., Ebel G.D., Armstrong P.M., Barker C.M, & Smith R.C. (2022). Semi-field and surveillance data define the natural diapause timeline for Culex pipiens across the United States. Communications Biology, 5, 1300.
Other organizations :
Iowa State University, Connecticut Agricultural Experiment Station, Consolidated Mosquito Abatement District, Pennsylvania Department of Environmental Protection, Vector (United States), Suffolk University, Colorado State University, University of California, Davis
Rearing conditions: diapause (19 °C, 9:15 L:D) and control (25 °C, 16:8 L:D)
dependent variables
Differences in lipid stores of adult females (6–9 days old)
control variables
Adult female age (6–9 days old)
Staining with Nile Red (1:100 PBS dilution of 500 μg/ml Nile Red powder in acetone stock)
Incubation time (10 min)
Imaging using a Nikon 50i fluorescent microscope and Nikon imaging software (NIS Elements D 3.2)
Annotations
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