Electrospun PCL Scaffolds for Tissue Engineering

PCL was dissolved in acetone at 10% and 15% (w/v) under agitation at 60 °C for 24 h. Scaffolds were manufactured by an electrospinning machine (Spraybase, Dublin, Ireland). The solution was moved to a 20 mL syringe connected to a stainless steel 18G needle (inner diameter of 0.8 mm) through a polytetrafluoroethylene tube (inner diameter of 1 mm). The distance between the emitter and collector was 10 cm. The electrospinning process was established by Syringe Pump Pro software (New Era Pump Systems; Farmingdale, NY, USA). For the 10% PCL concentration, 8.5 mL of solution was ejected at a voltage of 9 kV and a flow rate of 6 mL/h. For the 15% PCL concentration, 5 mL was ejected at a voltage of 7 kV and a flow rate of 6 mL/h. The resulting meshes were cut and sterilized as described elsewhere [38 (link)].