Lymph nodes were collected from Rag1−/− B3K506 TCR transgenic, Rag1−/− B3K508 TCR transgenic, and Rag1−/−UbcGFP TEa TCR transgenic mice and a small sample was stained with APC-labeled CD4 antibody (RM4–5, Tonbo biosciences) and analyzed on an LSR II (BD Biosciences) flow cytometer using Flowjo software (TreeStar). The results were used to calculate the amount of the remaining sample needed to transfer one million CD4+ T cells. In some cases, the T cells from the Rag1−/− B3K506 and Rag1−/− B3K508 TCR transgenic mice were also labelled with CellTracker Orange (ThermoFisher Scientific) or CellTraceViolet (ThermoFisher Scientific), respectively (19 (link)). One million TCR transgenic cells were transferred into B6 mice by i.v injection 24 h prior to infection with Lm-P5R.
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Adoptive Transfer of TCR Transgenic T Cells
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Corresponding Organization : University of Minnesota
Other organizations : Imaging Center
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Variable analysis
independent variables
- Genetic background of mice (Rag1-/- B3K506 TCR transgenic, Rag1-/- B3K508 TCR transgenic, and Rag1-/- Ubc-GFP TEa TCR transgenic)
- Cell labeling (CellTracker Orange, CellTraceViolet)
- Amount of CD4+ T cells collected from lymph nodes
- Time of T cell transfer (24 h prior to infection with Lm-P5R)
- Route of T cell transfer (i.v. injection)
- None specified
- None specified
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