Genetic Mouse Models of Bone and Cancer

Col2a1-creERT2,⁸ Osx-creERT2,^{3 (link)} Col1-creERT2,^{9 (link)} and Cre-dependent Kras transgenic, loxP-stop-loxP-Kras^{G12D10 (link)} mice have been described elsewhere. The Cre reporter, Rosa26-loxP-stop-loxP-tdTomato mice were acquired from Jackson Laboratory (Bar Harbor, ME, USA).^{7 (link)} All procedures were conducted in compliance with the Guideline for the Care and Use of Laboratory Animals approved by Massachusetts General Hospital's Institutional Animal Care and Use Committee (IACUC). Mice were in a mixed genetic background. Comparisons were made between littermates. Tamoxifen injections were given i.p. at a dose of 0.1 mg/g to pregnant females as described elsewhere.^{4 (link)}