Human renal carcinoma 786-O cells were cultured in RPMI-1640 media (GIBCO, NY, USA) supplemented with 10% fetal bovine serum (Hyclone, MA, USA) at 37°C and 5% CO2. Irradiation was carried out by laboratory X-ray source (RX-650, Faxitron Bioptics, USA) [27 (link)]. The dose rate was 0.8 Gy/min (100 keV, 5 mA). Cells were seeded into 12-well plates and grown to <70% confluence at the time of irradiation.
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