Lipid and Glucose Metabolism Assessment

The lipid profile including total-cholesterol, triglyceride (TG), and free fatty acid (FFA) were measured using a Hitachi 7600 autoanalyzer (Hitachi Ltd., Tokyo, Japan). For separating HDL-cholesterol by dextran-sulfate magnesium precipitation the enzymatic method was used. The LDL-cholesterol concentration was calculated by the Friedewald equation. Lipoprotein-associated phospholipase A₂ (Lp-PLA₂) activity was measured with a high-throughput radiometric activity assay [21 (link)]. The fasting glucose level was analyzed by hexokinase method with a Hitachi 7600 autoanalyzer. Insulin level was measured with immunoradiometric assay, using commercial kit provided by Immuno Nucleo Corporation (Stillwater, MN, USA) and IR was calculated by homeostasis-model assessment (HOMA).

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Kim M., Lee S.H, & Lee J.H. (2016). Global Metabolic Profiling of Plasma Shows that Three-Year Mild-Caloric Restriction Lessens an Age-Related Increase in Sphingomyelin and Reduces L-leucine and L-phenylalanine in Overweight and Obese Subjects. Aging and Disease, 7(6), 721-733.

Publication 2016

Hitachi 7600 autoanalyzer

Cholesterol Dextran Dextran sulfate Enzymatic Free fatty acid Glucose Hdl cholesterol Hexokinase High throughput assay Homeostasis Immunoradiometric assay Insulin Ldl cholesterol Lipid Lipoprotein associated phospholipase a2 Magnesium Magnesium sulfate Radiometric Triglyceride

Corresponding Organization :

Other organizations : Yonsei University, National Health Insurance Service

Top 5 similar protocols

Variable analysis

independent variables

Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity

dependent variables

Total-cholesterol
Triglyceride (TG)
Free fatty acid (FFA)
HDL-cholesterol
LDL-cholesterol
Fasting glucose level
Insulin level
IR (calculated by homeostasis-model assessment)

control variables

The Hitachi 7600 autoanalyzer (Hitachi Ltd., Tokyo, Japan) used for measuring lipid profile and fasting glucose level
The enzymatic method used for separating HDL-cholesterol by dextran-sulfate magnesium precipitation
The Friedewald equation used for calculating LDL-cholesterol concentration
The high-throughput radiometric activity assay used for measuring Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity [21]
The hexokinase method used for analyzing the fasting glucose level with the Hitachi 7600 autoanalyzer
The immunoradiometric assay using a commercial kit provided by Immuno Nucleo Corporation (Stillwater, MN, USA) for measuring insulin level

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