Hydrogel Stiffness Characterization by AFM

Stiffness of the hydrogels was confirmed by atomic force microscopy (AFM; MFP3D, Asylum Research) as detailed previously.^{[18 (link),9 (link),20 ]} Briefly, a pyramidal probe, 0.08 N/m spring constant with a 35° half angle (PNP-TR20, Nanoworld), was used to indent the substrate. The probe indentation velocity was fixed at 2 μm/s with the trigger force of 2 nN. Elastic modulus maps were determined by the Hertz cone model with a sample Poisson ratio of 0.5 fit over a range of 10%-90% indentation force.²⁷ AFM software (Igor pro 6.22) was applied to generate the stiffness.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Hribar K.C., Finlay D., Ma X., Qu X., Ondeck M.G., Chung P.H., Zanella F., Engler A.J., Sheikh F., Vuori K, & Chen S. (2015). Nonlinear 3D Projection Printing of Concave Hydrogel Microstructures for Long-Term Multicellular Spheroid and Embryoid Body Culture. Lab on a chip, 15(11), 2412-2418.

Publication 2015

MFP3D PNP-TR20

Atomic force microscopy Cone Hydrogels Indent Maps Trigger

Corresponding Organization : University of California, San Diego

Other organizations : Sanford Burnham Prebys Medical Discovery Institute, Sanford Consortium for Regenerative Medicine

Top 5 similar protocols

Variable analysis

independent variables

Indentation force (10%-90%)

dependent variables

Elastic modulus

control variables

Pyramidal probe with 0.08 N/m spring constant and 35° half angle
Indentation velocity of 2 μm/s
Trigger force of 2 nN
Sample Poisson ratio of 0.5

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!