Immunohistochemical Analysis of TCam-2 Cells

Immunohistochemistry (IHC) was performed as published previously [10 (link)]. Tumor tissues were dissected, fixed in 4 % formalin overnight and processed in paraffin wax. Signal detection was performed semiautomatically in the Autostainer 480 S (Medac, Hamburg, Germany). Nuclei were stained by hematoxylin. See Table S1 for antibody details and dilution ratios. For IHC, 5 tumor tissues from TCam-2-ΔSOX2 clones and 1 tumor tissue from 2102EP cells as control was analyzed.

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Nettersheim D., Heimsoeth A., Jostes S., Schneider S., Fellermeyer M., Hofmann A, & Schorle H. (2016). SOX2 is essential for in vivo reprogramming of seminoma-like TCam-2 cells to an embryonal carcinoma-like fate. Oncotarget, 7(30), 47095-47110.

Publication 2016

Autostainer 480 S

Antibody Cells Clones Dilution Formalin Immunohistochemistry Nuclei Paraffin wax Signal detection Tissue Tumor

Corresponding Organization : University of Bonn

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Variable analysis

independent variables

Type of tumor tissues (TCam-2-ΔSOX2 clones and 2102EP cells)

dependent variables

Protein expression (detected by immunohistochemistry)

control variables

Fixation of tumor tissues in 4% formalin overnight
Processing of tumor tissues in paraffin wax
Signal detection method (semiautomated in Autostainer 480 S)
Nuclei staining by hematoxylin

controls

Positive control: 1 tumor tissue from 2102EP cells
Negative control: Not explicitly mentioned

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