Plasmid and siRNA Transfection Assay

pcDNA3.1/XPC-V5-His plasmid containing XPC cDNA was described previously [47 (link)]. XPC shRNA (short hairpin RNA) were purchased from Sigma (TRCN0000083118, TRCN0000083119). XPC siRNA and a scramble non-targeting siRNA (siCtrl) were purchased from Dharmacon (siGENOME human XPC siRNA SMARTpool, siXPC-#1: 5′-GCA AAU GGC UUC UAU CGA A-3′, siXPC-#2: 5′-GGA GGG CGA UGA AAC CUU U-3′, siCtrl: 5′-UUC UCC GAA CGU GUC ACG U-3′). pcDNA3/E-Cadherin plasmid containing E-Cadherin cDNA (NM_004360.3) was purchased from Addgene (Addgene plasmid #45769, ref: [49 (link)]) The plasmids and siRNA were transfected into cells by using Lipofectamine 2000 reagents (Life Technologies) according to manufacturer's instructions.