Multivariate Analysis of Cadmium Contamination

When performing the statistical analysis, 41 samples were divided into 6 groups, which were branch river control (n = 3), upstream control (n = 6), sewage outfall (n = 6), dosing sites (n = 7), midstream (n = 16), and farmland (n = 2) (Table S1). The physicochemical indices were statistically analysed with separate one-way analyses of variance (ANOVA). The correlations (correlation between Cd content of sludge and Cd concentration of river water; the correlation between Cd content of five different phases in sludge and the total Cd content in sludge; the correlation between Cd content in sludge and microbial richness (Chao1 index); the correlation between Cd content in sludge and the alpha diversity (Shannon index) of the communities in the sludge samples; the correlation between Cd content in sludge and microbial structure) were analysed with bivariate correlation, and these statistical analysis were performed using SPSS 19.0 for Windows^{81 (link)}. To evaluate the effects of environmental factors on overall functional community structures, CCA was implemented with the CANOCO 4.5 software package^{82 (link)}.

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Zhang M., Huang F., Wang G., Liu X., Wen J., Zhang X., Huang Y, & Xia Y. (2017). Geographic distribution of cadmium and its interaction with the microbial community in the Longjiang River: risk evaluation after a shocking pollution accident. Scientific Reports, 7, 227.

Publication 2017

SPSS 19.0 for Windows

River Sewage Sludge

Corresponding Organization :

Other organizations : General Research Institute for Nonferrous Metals (China), Institute of Scientific and Technical Information

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Variable analysis

independent variables

Location (branch river control, upstream control, sewage outfall, dosing sites, midstream, farmland)

dependent variables

Physicochemical indices
Cd content of sludge
Cd concentration of river water
Cd content of five different phases in sludge
Total Cd content in sludge
Microbial richness (Chao1 index)
Alpha diversity (Shannon index) of the communities in the sludge samples
Microbial structure

control variables

Number of samples in each group (branch river control (n = 3), upstream control (n = 6), sewage outfall (n = 6), dosing sites (n = 7), midstream (n = 16), farmland (n = 2))

positive controls

Not specified

negative controls

Not specified

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