Hypoxia-Conditioned MSC Secretome Enhances HUVEC

Hypoxia is a pathophysiologic consequence of vascular and perfusion deficits [14 (link), 15 (link)]. Hypoxia has also been shown to enhance the proangiogenic properties of the MSC secretome [16 (link)–19 (link)]. Thus, we determined whether sbMSCs conditioned media in the setting of hypoxia could also promote human umbilical vein endothelial cell (HUVEC) survival and migration. To generate hypoxic conditioned media, sbMSCs (n = 4 patients) were cultured in T75 flask with 6 mL of basal EGM-2 media at 1% O₂ using a hypoxic incubator (Eppendorf Inc., Enfield, CT). Conditioned media was then collected at 48 hours and stored at −80°C until used in downstream experiments.