Surgical Induction of Myocardial Infarction in Mice
Corresponding Organization :
Other organizations : University of Washington
Variable analysis
- Ligation of the left anterior descending artery
- Sham operation (no coronary artery ligation)
- Survival of mice beyond the first 24 hours after surgery
- Male 8- to 12-week-old mice weighing 22–28 g
- Anesthesia with 4% isoflurane and ventilation with 2% isoflurane at 130 breaths per minute
- Small skin cut (~1.2 cm) over the left chest
- Dissection and retraction of the pectoral major and minor muscle
- Exposure of the fourth intercostal space
- Popping out the heart through the hole in the fourth intercostal space
- Suturing and ligation of the left coronary artery at a site about 3 mm from its origin using a 7-0 Ethilon silk suture
- Manual evacuation of air and closure of muscle and skin
- Sham-operated animals underwent the same procedure without coronary artery ligation
- Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!