Before imaging, samples were mounted in STORM imaging buffer, consisting of Tris (50 mM; pH 8.0), NaCl (10 mM), glucose (10 % wt/vol), β-mercaptoethanol (143 mM; Sigma, M3148), and an enzymatic oxygen scavenger system (1% vol/vol). The enzymatic oxygen scavenging system was added to the buffer immediately before use, and the 100× stock solution was prepared by mixing pyranose oxidase powder43 (link) (10 mg; Sigma, P4234) with catalase slurry (80 μl; Sigma, C100) in PBS (170 μl), and centrifuging the mixture at 13,000 r.p.m. for 1 min.
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