p120-Catenin and F-actin Co-immunostaining

p120-Catenin/F-actin co-immunostaining was performed as already described^{48 (link)}. Cells were rinsed, fixed, permeabilized and primary rabbit monoclonal anti-p120 catenin antibody (Abcam, ab92514) (1/100 final) was added and incubated for 1 h at room temperature. Corresponding secondary antibody (Alexa Fluor 568-conjugated antibody, ThermoFisher Scientific, ref A11036) (1/250 final) was added for 1 h at room temperature. After rinsing, 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (ThermoFisher Scientific, D1306) staining was performed for 10 min at room temperature at 0.2 µg/mL (final concentration). After rinsing, slides were mounted in Vectashield mounting medium without DAPI (Eurobio/Abcys).