The leaves of the M. azedarach plant were prepared as previously described [29 (link)]. The leaves were thoroughly washed with distilled water soon after collection and blotted dry using a paper towel. Approximately 10 g of the leaves were cut into small pieces, transferred to a beaker containing 40 mL of 18 Mohm water, and heated to between 80 and 90 °C, with constant stirring for 15 min, until a green-colored liquid solution was noted. The leaf extract was filtered (Whatmann Filter paper no. 5) and stored in a freezer at −4 °C until further use.
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