Bacterial Catalase Activity Assay

Bacterial strains were grown overnight at 37 °C with 250 rpm shaking in LB broth into lag phase prior to the assay in a total volume of 5 mL in a 50 mL conical tube (Sarstedt, Montréal, QC, Canada). Absorbance at 600 nm was measured prior to the assay for the purpose of normalization of the catalase activity based on the growth of each strain. The catalase activity assay was carried out using a Gilson oxygraph equipped with a Clark electrode as described previously [72 (link)]. The assay was carried out in biological duplicates and the average values with the standard deviations were plotted and statistically analyzed using GraphPad Prism v6.07 (La Jolla, CA, USA).