A blood sample was collected after an overnight fast of >8 h. Plasma glucose levels were measured using a hexokinase enzymatic reference method. Fasting insulin levels were measured using a radioimmunoassay (RIA) method (Coat A Count Insulin, Los Angeles, USA). Fasting lipids were analyzed, and for the present study serum levels of cholesterol ≥5.172 mmol l-1 and triglycerides ≥1.7 mmol l-1 were considered abnormal.
HOMA-IR was used to evaluate insulin resistance (fasting serum insulin (μU/ml) × fasting plasma glucose (mmol l-1)/22.5) [27 (link)].
HOMA-IR was used to evaluate insulin resistance (fasting serum insulin (μU/ml) × fasting plasma glucose (mmol l-1)/22.5) [27 (link)].
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