Rab7 antibody (Cell Signaling Technology Cat# 2094, RRID:AB_2300652) was used at a dilution of 1:200 in 10% BSA. This antibody has been validated after siRNA-mediated knockdown on western blots19 (link). It has additionally been validated after knockdown and immunofluorescent staining20 (link). Validation after knockdown was also carried out in our lab for neuronal cultures (data not shown). Rab11 antibody (Cell signaling technology, Cat# 3539 S, RRID: AB_2253210) was used at a dilution of 1:200. This antibody was validated by siRNA knockdown and western blot21 (link). It has additionally been validated through knockdown and immunofluorescent staining22 . EEA1 antibody (Cell Signaling Technology Cat# 2411 S, RRID:AB_2096814), was used at a dilution of 1:200. This antibody staining is consistent with early endosomes, co-localizing with Transferrin, but not Rab723 (link). In our hands, this antibody recognized overexpressed tagged EEA124 (link), co-localizes with endocytosed Tfn at short but not long chase times, the staining does not co-localize with degradative cargos, and the staining disappears when early endosomes are disrupted by Rab5 interference25 (link).
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