Vacuolin-1 and Apilimod Inhibit Lysosome Function

Vacuolin-1 (18 (link)) was custom synthesized; Apilimod (HY-14644) was from MedChem Express, IN1 was a kind gift from N. Gray, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA (33 (link)), U-18666A (10009085) and Filipin III (70440) were from Cayman Chemical, Bafilomycin A1 (B1793-2UG) was from Sigma-Aldrich, Cycloheximide (239764) was from Calbiochem, and wheat germ agglutinin conjugated with Alexa Fluor-647 (W32466) was from ThermoFisher.

Free full text: Click here

Kang Y.L., Chou Y.Y., Rothlauf P.W., Liu Z., Soh T.K., Cureton D., Case J.B., Chen R.E., Diamond M.S., Whelan S.P, & Kirchhausen T. (2020). Inhibition of PIKfyve kinase prevents infection by Zaire ebolavirus and SARS-CoV-2. Proceedings of the National Academy of Sciences of the United States of America, 117(34), 20803-20813.

Publication 2020

Apilimod Filipin III Bafilomycin A1 (B1793-2UG)

Alexa fluor 647 Apilimod Bafilomycin a1 Cancer Cayman Cycloheximide Filipin iii U 18666a Vacuolin 1 Wheat germ agglutinin

Corresponding Organization :

Other organizations : Harvard University, Boston Children's Hospital, Washington University in St. Louis, Boehringer Ingelheim (United States)

Top 5 similar protocols

Variable analysis

independent variables

Vacuolin-1
Apilimod
U-18666A
Filipin III
Bafilomycin A1
Cycloheximide
Wheat germ agglutinin conjugated with Alexa Fluor-647

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!