For Western blotting experiments, we collected individual worms by hand and boiled them in Laemmli protein sample loading buffer prior to gel separation. Specific proteins were analyzed by western blotting with ECL detection (GE Healthcare) of HRP-coupled secondary antibodies (Jackson ImmunoResearch). Worm protein extracts for sucrose gradients and ribosome immunoprecipitations were made as described (5 (link)). The sucrose gradient centrifugation and fractionation was conducted as previously described (10 (link)).