Protein Extraction and Western Blotting for Worms
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Corresponding Organization : Martin Luther University Halle-Wittenberg
Other organizations : Max Planck Institute of Biochemistry
Variable analysis
- Collecting individual worms by hand
- Specific proteins analyzed by western blotting with ECL detection
- Worm protein extracts for sucrose gradients and ribosome immunoprecipitations
- Boiling worms in Laemmli protein sample loading buffer prior to gel separation
- Sucrose gradient centrifugation and fractionation as previously described
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
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