The adult zebrafish heads for histological analysis were prepared as described in (Stundl et al., 2023 (link)); briefly, the samples were rinsed in distilled water, decalcified in Morse’s solution, and embedded into the JB4 resin (prepared according to the manufacturer’s instructions, Sigma-Aldrich) at room temperature overnight. The next day, the infiltration solution was replaced by an embedding solution (prepared according to the manufacturer’s instructions), placed into an embedding mold (Polyscience), and transferred to the vacuum chamber, which accelerated the polymerization (~3 hours). The resin block was sectioned at 7 μm, and sections were stained with Mayer’s hematoxylin.
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