Measuring Cell Impedance Dynamics

Cell function (hMEC) was assessed by changing of electrical impedance measured by the xCELLigence system, using the RTCA SP instrument (Roche Applied Science) as previously described^{42 (link)}. Briefly, cells at a density of 2 × 10⁴ per well were plated in a 96 well E-plate containing gold microelectrode sensors on the bottom to measure cellular impedance inside each well. Cells were allowed to adhere overnight and after were treated with rS1p for 60 min. Values are represented by cell index numbers, which are a dimensionless unit of measurement representing the measurement of zero impedance when cells are absent and increasing as cells adhere, spread and divide, and analysed using RTCA software (Roche). The slope was measured based on the normalised cell index from the point in which rS1p treatment was administered.

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Montezano A.C., Camargo L.L., Mary S., Neves K.B., Rios F.J., Stein R., Lopes R.A., Beattie W., Thomson J., Herder V., Szemiel A.M., McFarlane S., Palmarini M, & Touyz R.M. (2023). SARS-CoV-2 spike protein induces endothelial inflammation via ACE2 independently of viral replication. Scientific Reports, 13, 14086.

Publication 2023

RTCA SP instrument RTCA software

Cell Cell function Gold Microelectrode Rtca

Corresponding Organization : McGill University

Other organizations : University of Glasgow, MRC University of Glasgow Centre for Virus Research

Top 5 similar protocols

Variable analysis

independent variables

RS1p (treatment)

dependent variables

Electrical impedance
Cell index

control variables

Cell density (2 × 10^4 per well)
Overnight cell adherence

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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